Go to OSU College of Forestry LWGate Home Page.From: Lloyd.Donaldson@forestresearch.co.nz
Subject: Re: [IAWA Forum] Disposable microtome knives
Date: Mon, 24 Mar 2003 12:33:42 +1200
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Hi Jenny
At that sort of density you will have a major problem sectioning with any
sort of tool. In my experience disposable knives (Feather brand) will work
on softened material upto about 7-800 kg m3. In your case you might try to
investigate the use of milling devices to prepare the sample. CSIRO use a
diamond mill to prepare cores for silviscan which might work on your
material. I suggest you contact Geoff Downes or Jugo Ilic. You could also
consider ion beam milling - contact Prof. Mel Dickson at University of NSW.
M.Dickson@unsw.edu.au You could also contact Jim Darvey at Proscitech in
Brisbane - they sell a range of disposable knives and might be able to
suggest something.
http://www.proscitech.com.au
Depending on how much you can soften the sample it may be a case of getting
a smooth surface for SEM rather than actually sectioning.
Good luck !
Regards
Dr Lloyd Donaldson - Microscopist
Cellwall Biotechnology Centre
Forest Research,
Private Bag 3020, Rotorua, New Zealand
Ph 64 7 343 5581
email lloyd.donaldson@forestresearch.co.nz
www.forestresearch.co.nz/mnz
"Jennifer
Barker" To: iawa@COF.ORST.EDU
<jenpenten@hot cc:
mail.com> Subject: [IAWA Forum] Disposable microtome knives
Sent by:
owner-iawa@COF
.ORST.EDU
24/03/03 11:23
AM
Please respond
to "Jennifer
Barker"
Dear list-members
Can anyone out there recommend a brand of disposable microtome knives
suitable for a sledge microtome and for sectioning (softened) wood with a
density up to 1400 kg/m3 (86lbs/ft3)?
Many thanks in advance.
Jenny Barker
PhD Candidate
Environmental Biology
(Benham Building)
School of Earth & Environmental Sciences
Adelaide University
North Terrace
Adelaide SA 5005
Rm 119 Benham
(08) 8303 3088
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